Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.430754
Title: Genetic aberrations in high-grade astrocytic gliomas
Author: Mulholland, Paul James
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 2006
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Abstract:
Astrocytic gliomas are the most common tumours arising in the central nervous system. This thesis focuses on examining genetic changes in high-grade astrocytic gliomas. It describes the development of an analytical tool to facilitate systematic analysis of array comparative genomic hybridisation (CGH) results and catalogues copy number changes in high-grade astrocytic glioma cell lines and patient samples. Molecular cytogenetic analysis of chromosome 6 was used to explore the relationship between copy number changes and positional aberrations. Three regions of copy number associated with translocation breakpoints are described. The first translocation associated with a copy number change was in the cell line U87, loss of 6pter to 6p21 arises from an unbalanced translocation between chromosomes 6 and 7, with the breakpoint at 6p21 being close to the POLH and GTPBP2 genes. The second is a discrete deletion in 6q23- 24 in U87 is associated with a translocation between chromosomes 6 and 12, and involves several genes including MAP3K5 and MAP7. The third is a discrete deletion of 6q26-27 in cell lines CRL2020 and CRL1620 involving IGF2R, PARK2, PACRG and QKI is associated chromosome translocations. Inactivation of a tumour suppressor gene near the breakpoint may be a primary consequence of certain translocations in solid tumours, in contrast to the oncogenic gene fusions present in haematological and mesenchymal malignancies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.430754  DOI: Not available
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