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Title: Properties of the nicotinic acid adenine dinucleotide phosphate-binding protein in sea urchin eggs
Author: Churamani, Dev
ISNI:       0000 0001 3551 2792
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 2006
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Nicotinic acid adenine dinucleotide phosphate (NAADP) has recently emerged as a novel intracellular calcium mobilising messenger in a variety of cells. Whilst increasing evidence suggests that NAADP acts on a distinct binding protein, little is known regarding the biochemical properties of the putative NAADP "receptor". My thesis investigates properties of the NAADP-binding protein in sea urchin eggs. Firstly, I show that NAADP binding to its target protein is inhibited by altering the protein:lipid ratio of soluble sea urchin egg homogenates - an effect prevented and reversed specifically by addition of exogenous phospholipids. These data highlight the importance of the lipid environment in maintenance of NAADP binding to its target protein. In addition, I show that upon binding its ligand, the NAADP-binding protein undergoes an unusual stabilization process that is dependent upon the time the receptor is exposed to its ligand. This property endows the NAADP-binding protein with the extraordinary ability to detect the duration of its activation. Finally I describe the development of a highly sensitive radioreceptor assay (based upon the sea urchin egg NAADP-binding protein) that is capable of detecting low levels of NAADP from cellular extracts. I apply this technique to determine NAADP levels in a variety of extracts prepared from cells under resting and stimulated conditions.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available