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Title: Proteomic analysis of secreted proteins from Phytophthora infestans
Author: Li, Shuang
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2004
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In order to identify potential avirulence factors of Phytophthora infestans, secreted protein profiles of six strains (IPO-0, CBS431.90, IPO-655-2A, IPO-428-2, 88069 and 90128) with different avirulence phenotypes were analysed by proteomics.  The proteins from culture filtrates were visualised on 2D gels with a total number between 350 and 1000.  PiAVR X was found only in avirulent strains and considered therefore as a possible avirulence determinant.  Its expression increased in all strains tested when grown in Modified Henniger medium.  Single nucleotide polymorphisms (SNPs) were detected within a 735 nt fragment of PiAvrX but none was linked to avirulence characteristics.  The consensus EST database facilitated identification of approximately 50% of 45 most abundant proteins excised from strain 88069.  Beta-glucosidase, Pi-NIP2 (Phytophthora infestans necrosis inducing protein-like protein), enoyl CoA hydratase, glutathione S transferase, peptidylprolyl isomerase, acidic chitinase and Pi-PR1 (Phytophthora infestans homologue of plant PR-1 protein) were among the identified proteins with a recognisable signal sequence.  Enolase, quinine-oxido reductase, nucleoside di-phosphate kinase, actin depolymerisation factor, thioredoxin, ubiquitin and 14-3-3 protein were also identified but without a signal peptide.  The occurrence and expression of Pi-nip2, Pi-chi1 and Pi-pr1 were confirmed in the strains tested.  Transformants were obtained from P. infestans strain T30-4 via a biolistic particle delivery approach using a single plasmid vector containing Pi-pr1.  Detailed analysis of these transformants did not demonstrate induction of homology-dependent gene silencing.  It was found that transformation rates were different among the tested P. infestans strains.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Phytophthora infestans