Novel stains as malaria diagnostics
Chapter One highlights the importance of the control of malaria and its significance to the health of the world population. The vital role of the diagnosis of malaria is discussed as are the advantages and disadvantages of the different diagnostic techniques. This Chapter also provides a brief introduction to the chemistry of synthetic dyes. Chapter Two reviews the mechanism of synthesis of cyanine dyes, as these are of particular importance in this research work. The characteristic properties of these dyes are discussed. Chapter Three deals with the spectroscopic properties of the dyes synthesised in this work. Chapter Four studies the use of theoretical calculations for the characterisation of the electronic and structural properties of the dyes as well as the relation of such properties to their biological activity. Chapter Five examines the effectiveness of the biological activity of the dyes as malaria diagnostics. The complex of dye molecule and DNA can be explained in terms of an intercalculation model. Chapter Six studies the redox behaviour of the dyes with complex metal hydrides, catalytic hydrogenation, and hydrogen peroxide. The electrochemical reduction/oxidation using cyclic voltammetry is described.