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Title: The immune response of the mouse to Diplostomum phoxini and certain cestodes in the intestinal lumen
Author: Bowen, David Huw
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1984
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Abstract:
This thesis was Concerned with the study of gut immunity to an intestinal trematode. Me parasite used was the strigeid Diplostomum phoxini. Adult D, phoxini normally parasitise the intestine of fish eating birds, but will readily establish and reach sexual maturity in laboratory infected mice. After reaching sexual maturity in about 4 dayst the'worms are lost shortly afterwards, and there is strong evidence that this loss is immunologically mediated by the host. The aims of this thesis were to establish the kinetics of the infection in mice and to investigate aspects of the immune response of the host to the parasite. The first chapter was concerned with the kinetics of establishment and rejection of both a Drimary and secondary infection of D. Rhoxini. The kinetics of heavy (200 metacercariae) and light (20 metacercariae) infections were com-Dared in an attempt to economise on the number of parasites required, especially with regards to immunization schedules. Results showed that in NIH mice, at least 80% of the administered worms would establish in the intestine. This wom T)opulation remained stable until rejectiong the onset of which was, related to the level of infection, i. e. heavy worm burdens were expelled earlier than light infections. The most important experiment with regards to immunity was the demonstration that a secondary infection was ex-oelled earlier than a primary infection, showing that loss was Drobably immunologically mediated and that 'memory cells" were established as a result of a primary infection in a mouse.This thesis was Concerned with the study of gut immunity to an intestinal trematode. Me parasite used was the strigeid Diplostomum phoxini. Adult D, phoxini normally parasitise the intestine of fish eating birds, but will readily establish and reach sexual maturity in laboratory infected mice. After reaching sexual maturity in about 4 dayst the'worms are lost shortly afterwards, and there is strong evidence that this loss is immunologically mediated by the host. The aims of this thesis were to establish the kinetics of the infection in mice and to investigate aspects of the immune response of the host to the parasite. The first chapter was concerned with the kinetics of establishment and rejection of both a Drimary and secondary infection of D. Rhoxini. The kinetics of heavy (200 metacercariae) and light (20 metacercariae) infections were com-Dared in an attempt to economise on the number of parasites required, especially with regards to immunization schedules. Results showed that in NIH mice, at least 80% of the administered worms would establish in the intestine. This wom T)opulation remained stable until rejectiong the onset of which was, related to the level of infection, i. e. heavy worm burdens were expelled earlier than light infections. The most important experiment with regards to immunity was the demonstration that a secondary infection was ex-oelled earlier than a primary infection, showing that loss was Drobably immunologically mediated and that 'memory cells" were established as a result of a primary infection in a mouse.IgM was specific anti worm antibody. The final cha-nter was-concerned with the non-specific responses of the mouse. The intra epithelial globule leukocyte, lamina T)roDoria mast cell and goblet cell resnonse to infection was investigated. Both the intra epithelial globule leukocytes and lamina Droporia mast cells increased in numbers in response to infection, with the greatest resnonse observed in the intra epithelial globule leukocyte DoDulation. Wo increase in goblet cell numbers was observed. The transfer of immune mesenteric lymph node cells in the presence of D. phoxini also accelerated the increase in the intra eDithelial globule leukocyte and lamina Droporia mast cell DoDulations which suggests that these cells may be under lymphocyte control. Finally the results of the e"eriments on Do Rhoxini were discussed in relation to the more well known, but still far from well understood, nematode and cestode models.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.372400  DOI: Not available
Keywords: QR180 Immunology ; QL Zoology Human anatomy
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