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Title: Early mouse development.
Author: Murray, Patricia Ann.
ISNI:       0000 0001 2424 9095
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2001
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The five manuscripts presented here concern events that occur during the early stages of mouse development, beginning with differentiation of primitive endoderm and ending with formation of the proamniotic cavity. The main focus of the work has been the role of the basement membrane in early development, with particular reference to its role in the regulation of epiblast and extraembryonic endodermal cell differentiation, parietal endodermal cell migration and programmed cell death (manuscripts 1, 3 and 4). HOW"9ver,the critical role played by the basement membrane in these processes prompted an investigation into the mechanisms regulating the deposition of this specialised extracellular matrix (manuscript 2). Central to all four manuscripts has been the use of an extremely good in vitro model system, the embryoid body. Embryoid bodies are derived from embryonic stem cells of the mouse blastocyst, and their development closely resembles that of the periimplantation embryo. Of particular use, has been the availability of LAMC1-/- ES cells that are unable to express the laminin y1 chain (manuscript 5). This defect renders the cells incapable of assembling a functional laminin type-1 trimer, which is necessary for basement membrane deposition. Hence, embryoid bodies derived from LAMC1-/- ES cells lack basement membranes, allowing the role of the basement membrane in early development to be analysed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Genetics Molecular biology Cytology Genetics Biochemistry