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Title: Genetical studies on enzyme polymorphisms in Aedes aegypti
Author: Field, W. N.
Awarding Body: City of Birmingham Polytechnic
Current Institution: Birmingham City University
Date of Award: 1983
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Enzyme variants from strains of Ae. aegypti (L.) vere separated by polyacrylamide gel electrophoresis and detected by in situ staining procedures. Linkage relationships of an Est-6 locus in Isla Verde and Triple Marker strains vere characterised by using Bt , ~ and ~ as group II markers together vith !! and B!1 from groups I and III respectively. Est-6, vhich vas inherited in a Simple Mendelian fashion, vas located on linkage group II in relation to markers ~, ! and ~. Crosses performed betveen Triple Marker and London Susceptible strains using ~, ~, and Odh-1 as groups I, II and III markers respectively, indicated a linkage relationship betveen an Est-5, vhose products vere of next lover mobilities to~ose of Est-6, and !.!. Mechanisms responsible for high and lov Est-6 band intensity in the corresponding Cayenne and London Susceptible strains vere investigated. Backcross progeny, produced by reciprocally crossing the tvo parental strains and reciprocally backcrossing F, progeny vith London Susceptible, demonstrated discrete levels of band intensity indicative of a duplication system. Changes in esterase banding patterns, and therefore gene activity, during the ontogeny of Triple Marker selected strains, vere monitored using various substrates. An Est-6 band vas observed in all developmental stages; other bands vere rarely observed in adults. No bands vere found in egg or first instar larvae; nor vere all bands detected USing only one substrate. The es~erase pattern changes vere apparently not related to morphological changes. Esterase intensity, particularly that of Est-6, vas associated vith malathion resistance in adults of Villa Pa1meras and Triple Marker strains. This relationship vas used to design a test for assaying adult malathion resistance under field conditions. Electromorphic variation vas detected in alkaline phosphatases from Villa Palmeras and Triple Marker strains. Three putatively isozymic forms, each at least dimorphic for band occurrence and intensity, vere exhibited but no correlations vith malathion resistance vas observed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Genetics Molecular biology Cytology Genetics