Genetical studies on enzyme polymorphisms in Aedes aegypti
Enzyme variants from strains of Ae. aegypti (L.) vere separated
by polyacrylamide gel electrophoresis and detected by in situ staining
Linkage relationships of an Est-6 locus in Isla Verde and
Triple Marker strains vere characterised by using Bt , ~ and ~ as
group II markers together vith !! and B!1 from groups I and III
respectively. Est-6, vhich vas inherited in a Simple Mendelian
fashion, vas located on linkage group II in relation to markers ~,
! and ~.
Crosses performed betveen Triple Marker and London Susceptible
strains using ~, ~, and Odh-1 as groups I, II and III markers
respectively, indicated a linkage relationship betveen an Est-5,
vhose products vere of next lover mobilities to~ose of Est-6, and
Mechanisms responsible for high and lov Est-6 band intensity
in the corresponding Cayenne and London Susceptible strains vere
investigated. Backcross progeny, produced by reciprocally crossing
the tvo parental strains and reciprocally backcrossing F, progeny
vith London Susceptible, demonstrated discrete levels of band
intensity indicative of a duplication system.
Changes in esterase banding patterns, and therefore gene
activity, during the ontogeny of Triple Marker selected strains,
vere monitored using various substrates. An Est-6 band vas observed
in all developmental stages; other bands vere rarely observed in
adults. No bands vere found in egg or first instar larvae; nor
vere all bands detected USing only one substrate. The es~erase
pattern changes vere apparently not related to morphological changes.
Esterase intensity, particularly that of Est-6, vas associated
vith malathion resistance in adults of Villa Pa1meras and Triple
Marker strains. This relationship vas used to design a test for
assaying adult malathion resistance under field conditions.
Electromorphic variation vas detected in alkaline phosphatases
from Villa Palmeras and Triple Marker strains. Three putatively
isozymic forms, each at least dimorphic for band occurrence and
intensity, vere exhibited but no correlations vith malathion
resistance vas observed.