Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327535
Title: Inhibition of N-acyl-homoserine lactone quorum sensing
Author: Jones, Faye-Ellen
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2000
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Abstract:
Quorum-sensing amongst Gram-negative bacteria is an important method of intercellular communication required for conveying information about population density. The extracellular accumulation of the signal molecule involved, N-acyl homoserine lactone (AHL), leading to increases in internal physiological concentration, allows phenotypic switching to occur that is beneficial to the bacterial population. In our laboratory, analysis of the effect of AHL on phenotype currently involves creating null mutants unable to produce AHL, then reintroducing the signal molecule exogenously. With the increasing number of human, animal and plant pathogens utilising AHL quorum-sensing for regulating viirulence, AHLs have become prime targets for anti-infective therapy and crop-protection. The research described here has investigated methods of inhibition of quorum-sensing through the AHL signal molecule. These include the application of extremes of temperature and pH, and isolation and characterisation of the first recombinant human antibody fragment specific to AHLs from a naive phage display library, which could be used to examine cell-cell communication without the need for gene manipulation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.327535  DOI: Not available
Keywords: Intercellular communication Molecular biology Cytology Genetics Microbiology Biomedical engineering Biochemical engineering
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