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Title: Dictyostelid cellular slime moulds of the United Kingdom : isolation, identification and distribution.
Author: Hodgson, Helen.
Awarding Body: Anglia Polytechnic University
Current Institution: Anglia Ruskin University
Date of Award: 1999
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Soil samples were collected and processed, mainly from woodland, but also from non-woodland soils, particularly from designated Sites of Special Scientific Interest. The survey focussed on the East Anglian region and the north of England and Scotland, where Cavender & Raper's semi-quantitative isolation method was used to assess dictyostelid population densities at the sites surveyed. Qualititative species data only were also obtained from additional soil samples collected from within the United Kingdom. Isolation and identification methods were examined, and a total of 12 described species were recorded. Of these 12 species, 5 were the first reports for the United Kingdom, D. fasciculatum, D. polycarpum, P. candidum, D. implicatum and D. septentrionalis, the latter two being the first isolations of these species in Europe. In addition, one new species of Polysphondylium was isolated from soil collected from Silverdale Glen in the Isle of Man and a putative new Dictyostelium species was isolated from Thetford Forest, Norfolk. The variant, D. aureo-stipes var. helvetium, was also reported for the first time and one D. mucoroides variant was encountered at two sites. Images are presented ofall of these, together with a detailed description of the new species of Polysphondylium, and the characteristics of the putative new Dictyostelium isolate, together with measurements of both of these, which were obtained using image analysis. Allozyme electrophoresis was employed in an attempt to establish the affinities of the new species of Polysphondylium. A relational database was designed and constructed to facilitate identification of isolates and analysis of dictyostelid population densities. The data obtained during this survey were combined with that obtained by Turner (1978) to present a complete species distribution list of dictyostelids, to date, in the United Kingdom. A dictyostelid culture collection has been constructed, containing representatives of most of the strains encountered during this survey, which is available for future taxonomic study.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Ecology Ecology Soil science