Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae
This thesis examines the post-transcriptional regulation of the gluconeogenic mRNAs PCK1 and FBP1 in Saccharomyces cerevisiae. By construction of a set of chimaeric PCK1-PGK1 reporter genes, it was possible to show that sequences from the 5' end of the PCK1 mRNA were capable of conferring post-transcriptional glucose regulation on the reporter. These regions were presumably the ultimate target of the glucose signal on the wild-type PCK1 mRNA. Sequences from the 5' end of the SDH2 mRNA had previously been shown to be required for the rapid degradation of that transcript observed in the presence of glucose. In addition, a number of other elements within the PCK1 gene proved capable of constitutively destabilising the reporter mRNA. One of these bore similarity to a 70 bp mRNA destabilising element from the highly unstable MATα1 mRNA. Overall, the decay patterns of the chimaeric PCK1-PGK1 and FBP1-PGK1 mRNAs were consistent with other studies of the decay of chimaeric PGK1 mRNAs.