High-performance liquid chromatography and pharmacokinetics of some antibiotics
The principles of High Performance Liquid Chromatography
(HPLC) and pharmacokinetics were applied to the use of several
clinically-important drugs at the East Birmingham Hospital.
Amongst these was gentamicin, which was investigated over a
two-year period by a multi-disciplinary team. It was found
that there was considerable intra- and inter-patient variation
that had not previously been reported and the causes and
consequences of such variation were considered. A detailed
evaluation of available pharmacokinetic techniques was
undertaken and 1- and 2-compartment models were optimised with
regard to sampling procedures, analytical error and model-error.
The implications for control of therapy are discussed and an
improved sampling regime is proposed for routine usage.
Similar techniques were applied to trimethoprim, assayed
by HPLC, in patients with normal renal function and
investigations were also commenced into the penetration of drug
into peritoneal dialysate. Novel assay techniques were also
developed for a range of drugs including 4-aminopyridine,
chloramphenicol, metronidazole and a series of penicillins and
Stability studies on cysteamine, reaction-rate studies on
creatinine-picrate and structure-activity relationships in HPLC
of aminopyridines are also reported.
HPLC, pharmacokinetics, gentamicin, 4-aminopyridine,