Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280849
Title: A genetic approach to improvements of Candida tropicalis as a biocatalyst
Author: Perry, Patricia Mair
Awarding Body: Cranfield Institute of Technology
Current Institution: Cranfield University
Date of Award: 1990
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Abstract:
It is well documented in the literature that, in addition to carbon sources such as glucose, Candida species are able to utilise a wide variety of n-alkanes which are degraded via beta-oxidation. Prior to beta-oxidation a primary oxidation system acts on the end terminal methyl groups to generate carboxyl groups. The main industrial aim of the work was to optimise the bioconversion of pelargonate [CH3(CH2)7COOH] to azeleate [HOOC(CH2)7COOH], i.e. the primary oxidation steps, by blocking the beta-oxidation pathway in C. tropicalis. To this end, a library of Sau 3AI partially digested C. tropicalis NCYC997 genomic DNA in pBR322 was constructed from which it was hoped to isolate and disrupt the long-chain fatty acid acyl-CoA oxidase genes, POX4 and POX5, which catalyse the first step of beta-oxidation. The library was probed with oligonucleotides specific to POX4 and POX5, and a putative POX4 clone was isolated.
Supervisor: Warner, P. J. Sponsor: ChemGen Corporation
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.280849  DOI: Not available
Keywords: Microbiology Microbiology Molecular biology Cytology Genetics Biochemistry
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