Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274868
Title: Cell wall associated phenylpropanoids in fruit and vegetables
Author: O'Kennedy, Niamh
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2003
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Abstract:
Low molecular weight phenylpropanoid components of plant cell walls, ingested by humans as dietary fruit and vegetables, are released from covalent linkages to the cell wall by bacterial fermentation in the lower gut.  Based on their structural characteristics, such phenylpropanoids and their microbial metabolites potentially have the capacity to interact specifically with the cyclooxgenase (COX) enzymes, important mediators of the inflammation associated with developing neoplasia.  These cell wall components could therefore constitute an important dietary source of potentially bioactive compounds. A combination of enzymatic and chemical cell wall hydrolysis was used to liberate covalently bound low molecular weight phenylpropanoid derivatives from the polymer matrix of fruit and vegetable cell walls.  A range of ester- and ether-linked hydroxycinnamic and benzoic acids and derivatives, including significant quantities of dimeric 4-hydroxy-3-methoxycinnamates, was recovered from all samples examined.  An estimate of the bioavailability of these cell wall components was made by quantifying the proportion of measured cell wall phenylpropanoids released from the wall by enzyme digestion.  In approximately one half of the plant foods examined, these cell wall components were shown to occur at levels which represent a significant dietary burden (based on measured bioavailable compounds in a serving of 125 g plant fresh weight), and thus their presence in the diet is generally underestimated. The major cell wall associated hydroxycinnamates and the 4-hydroxy-3-methoxycinnamate dehydrodimers, as well as their major microbial metabolic products the phenylpropanoates, were examined for COX - inhibitory activity using an enzyme immunoassay.  Results showed a broad spectrum of activities, mainly non-specific.  Cell wall phenylpropanoid derivatives thus have the potential to alter the pattern of prostanoid formation in individuals consuming, as part of their diet, food plants with a high cell wall hydroxycinnamate content.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.274868  DOI: Not available
Keywords: Botany Botany Molecular biology Cytology Genetics
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