Schizophrenia genetics and DNA pooling
Genetic associations studies using both traditional and new approaches were performed on a sample of Scottish schizophrenics and controls. Three association studies were done using conventional individual genotyping, on the SCA1 and SCA6 triplet repeats and on the -141 cytosine insertion polymorphism of the Dopamine receptor 2 gene. No evidence was found to support the involvement of either triplet repeat; however, a modest effect on schizophrenia risk for the DRD2 variant was suggested by the data. The new approach of DNA pooling was then applied to schizophrenia genetics and reproducible, accurate methods developed for the analysis of microsatellites and other polymorphisms in DNA pools containing equal amounts of DNA from many individuals. These techniques were then applied to the screening of over a hundred microsatellites in three different areas of the gnome: chromosomes 13q, 8p and 22q. Pools of 200 Scottish schizophrenics and 200 controls were compared and identified 10 markers from these regions, which the results of this scan indicate are good candidates for further examination.