Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234531
Title: The biochemistry and physiology of poly-beta-hydroxybutyrate metabolism in Methylosinus trichosporium OB3b
Author: Williams, A. M.
Awarding Body: Cranfield University
Current Institution: Cranfield University
Date of Award: 1988
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Abstract:
The obligate methanotroph, Methylosinus trichosporium 0B3b synthesised up to 20% of its dry weight as poly-beta-hydroxybutyrate when grown in batch culture or nitrogen limited continuous culture on a methanol/ammonium salts medium. Four soluble enzymes associated with polymer metabolism, D(-)-3-hydroxybutyrate dehydrogenase (3-HBD), acetoacetyl-CoA synthetase, beta-ketothiolase and acetoacetyl-CoA reductase have been purified from the crude extract of this organism. Detailed kinetic studies were conducted on each enzyme and their role in the regulation of polymer metabolism was assessed. Cont/d.
Supervisor: Best, D. J. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.234531  DOI: Not available
Keywords: Methanotroph biochemistry
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